Фото: Влад Некрасов / Коммерсантъ
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This reaction would contain only low concentrations of the dNTPs and relied upon brief incubation times, so that DNA synthesis would stall at random positions along the template and yield a population of DNA fragments with varying lengths. These unfinished DNA fragments were then purified and used as templates in four “minus” and four “plus” reactions.，这一点在谷歌浏览器【最新下载地址】中也有详细论述
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